منابع مشابه
An Inactive Geminin Mutant That Binds Cdt1
The initiation of DNA replication is tightly regulated in order to ensure that the genome duplicates only once per cell cycle. In vertebrate cells, the unstable regulatory protein Geminin prevents a second round of DNA replication by inhibiting the essential replication factor Cdt1. Cdt1 recruits mini-chromosome maintenance complex (MCM2-7), the replication helicase, into the pre-replication co...
متن کاملRegulation of CDC6, geminin, and CDT1 in human cells that undergo polyploidization.
Endomitosis is the process by which mammalian megakaryocytes become polyploid during terminal differentiation. As in other endoreplicating cells, cyclin-cdk complexes are distinctly regulated, probably to overcome the strict mechanisms that prevent rereplication in most somatic cells. We have asked whether key factors involved in the assembly and licensing of replication origins are equally reg...
متن کاملInhibitory action of polyunsaturated fatty acids on Cdt1-geminin interaction.
A human replication initiation protein, Cdt1, is a central player in the cell cycle regulation of DNA replication, and geminin down-regulates Cdt1 function by direct binding. It has been demonstrated that Cdt1 hyperfunction resulting from Cdt1-geminin imbalance, for example, by geminin silencing with small interfering RNA, induces DNA re-replication and eventual cell death in some cancer-derive...
متن کامل3. A role for Cdt1 and Geminin in regulating genomic integrity and cancer promotion in tumor cells 4. Geminin and Cdt1 as markers for cancer prognosis 5. Perspectives: Geminin and Cdt1 as players in the molecular mechanisms of carcinogenesis
Cdt1 and its inhibitor Geminin are important regulators of replication licensing. In normal cells, a critical balance between these two proteins ensures that firing of each origin along the genome will take place only once per cell cycle. Cdt1 overexpression in cell lines and animals leads to aberrant replication, activates DNA damage checkpoints and predisposes for malignant transformation. Ge...
متن کاملGeminin Becomes Activated as an Inhibitor of Cdt1/RLF-B Following Nuclear Import
During late mitosis and early interphase, origins of replication become "licensed" for DNA replication by loading Mcm2-7 complexes. Mcm2-7 complexes are removed from origins as replication forks initiate replication, thus preventing rereplication of DNA in a single cell cycle. Premature origin licensing is prevented in metaphase by the action of geminin, which binds and inhibits Cdt1/RLF-B, a p...
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ژورنال
عنوان ژورنال: Genes
سال: 2015
ISSN: 2073-4425
DOI: 10.3390/genes6020252